SOURCE: Xtalks

Xtalks Webinars

February 25, 2015 07:05 ET

Characterization of Functional Methylomes by Next-Generation Capture Sequencing Identifies Novel Disease Associated Variants, New Webinar Presented by Roche NimbleGen, Hosted by Xtalks

TORONTO, ON--(Marketwired - February 25, 2015) - Join industry expert Dr. Elin Grundberg, Assistant Professor of Human Genetics at McGill University, for a live broadcast on Friday, March 13, 2015 at 10am EDT (2pm GMT).

Variation of DNA methylation is known to contribute to multifactorial disease susceptibility. However, current approaches for global methylome assessments require interrogation of the whole genome by next-generation sequencing (i.e. whole-genome bisulfite sequencing, WGBS), which is costly and inefficient due to invariable states of most sites across individuals, or are biased towards limited set of sites enriched in CpG-dense regions such as promoters (e.g. Illumina450K array or reduced representation bisulfite sequencing). To overcome these limitations, our featured speaker will present a new approach: MethylC-Capture Sequencing (MCC-Seq), for customizable and cost-effective sequencing of disease or tissue-targeted functional methylomes that simultaneously provides information on genetic variation.

This presentation will discuss how WGBS was used on adipose tissue samples and public databases to design a MCC-Seq adipose-specific panel. Its efficiency was established for high-density interrogation of methylome variability in all sequence contexts by systematic comparisons with other approaches, and demonstrated its applicability by identifying novel methylation variation within enhancers strongly correlated to plasma triglyceride and HDL cholesterol, including at CD36. The comprehensive adipose-panel allows parallel assessment of tissue methylation and genotypes at approximately 4M and 3M sites, respectively. The research that will be presented also demonstrates that MCC-Seq is as accurate as alternative epigenomics approaches but enables more efficient cataloguing of functional and disease-relevant epigenetic and genetic variants for large-scale disease association studies.

Since 2012 Dr. Grundberg has been an Assistant Professor at McGill University, where her group in the Department of Human Genetics is applying various next-generation sequencing approaches to understand genetic and epigenetic factors underlying complex disease susceptibility. She is the author of almost 50 scientific papers, and currently holds a Canada Research Chair (tier 2) in Disease Genomics and Epigenomics.

To learn more about this event visit: Characterization of Functional Methylomes by Next-Generation Capture Sequencing Identifies Novel Disease Associated Variants

Roche NimbleGen is an innovator in the life sciences market focused on research activities and manufacturing target enrichment probe pools for DNA sequencing. Capitalizing on the efficiencies inherent with parallel enrichment, researchers can now design economical, high throughput, and time-saving next-generation sequencing experiments. Next-generation sequencing combined with Sequence Capture probe pools offers researchers a clearer understanding of genomic structure and function in order to understand the impact of genes on biological processes. We are part of the innovations in sequencing solutions for life science research, now and in the future.

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global Life Sciences community. Every year thousands of industry practitioners (from pharmaceutical & biotech companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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