SOURCE: Xtalks

Xtalks Webinars

June 11, 2015 07:00 ET

Improved Methods for Structural Characterization of Therapeutic Proteins, New Webinar Hosted by Xtalks

TORONTO, ON--(Marketwired - June 11, 2015) - The presenters will discuss new solutions and strategies for streamlined glycosylation analysis. The live webinar takes place on Friday, June 19, 2015 at 1pm EDT.

In the areas of biotherapeutics, biomarker discovery, and glycoproteomics, it is essential to discriminate the protein and carbohydrate structures. Peptide-N-Glycosidase F (PNGase F) is a broad specificity amidase that liberates most N-linked glycans from mammalian glycoproteins. In recent years, PNGase F has become increasingly important in the biopharma industry, where defining the structure of N-glycans on therapeutic glycoproteins (e.g. antibodies) is imperative for defining their performance and quality control, and validating the structural equivalence of biosimilars. Additionally, PNGase F is increasingly being used in high throughput analyses of novel glycan-based markers of disease and development of diagnostics. The changing landscape of glycan analysis has placed new demands on the enzymes used for glycan removal with respect to their suitability for regulated environments and for high-throughput workflows.

Keynote speakers Dr. Alicia Bielik, Product Manager, Glycobiology at New England Biolabs, and Dr. Paula Magnelli, Scientist, Glycobiology at New England Biolabs, will introduce a series of novel products and techniques to facilitate glycoprotein analysis. First, an improved reagent, Rapid PNGase F, will be presented which allows for the complete deglycosylation of therapeutic monoclonal antibodies in just minutes. Validation of complete deglycosylation with Rapid PNGase F has been demonstrated using a variety of therapeutic monoclonal antibodies. Glycan labeling and clean up workflows have been optimized, allowing for the rapid preparation of glycans for analysis by LC-MS.

Dr. Bielik and Dr. Magnelli will also demonstrate the utility of several novel formulations of PNGase F, including an immobilized PNGase F suitable for solid-phase applications, a PNGase F fusion that can be easily removed from workflows, and a lyophilized PNGase F with exceptional stability. In addition, techniques using alternative endoglycosidases will be reviewed, such as Endo S, for the analysis of glycosylation of monoclonal antibodies. Finally, the use of exoglycosidases will be demonstrated, alone or in combination with endoglycosidases, for the study of complex protein mixtures, and / or proteins that are heavily N- and O-glycosylated.

To learn more about this complimentary event visit: Improved Methods for Structural Characterization of Therapeutic Proteins

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global Life Sciences community. Every year thousands of industry practitioners (from pharmaceutical & biotech companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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