SOURCE: Xtalks

Xtalks Webinars

June 24, 2015 07:00 ET

Post Translational Modification: Antibody Enrichment for Mass Spectrometry-Based Proteomics, New Webinar Hosted by Xtalks

TORONTO, ON--(Marketwired - June 24, 2015) - The one-hour live session taking place on Wednesday, July 15, 2015 at 11am EDT (4pm BST/ GMT +1), consists of two presentations followed by a Q&A with the audience. Featured speakers include Matthew Stokes, Principal Scientist of the Proteomics Group at Cell Signaling Technology, Inc., who will discuss PTMScan Proteomics, and Christopher Michael Rose, Postdoctoral Research Fellow in the Gygi Lab at Harvard Medical School, presenting on multiplexed quantitative ubiquitylome analysis.

PTMScan Proteomics: Identification and Quantification of Post-Translationally Modified Peptides

Protein post-translational modifications (PTMs) regulate all aspects of cellular growth, development, and disease biology. Proteomic analysis of these PTMs has become central to gaining a better understanding of cellular signaling. Current proteomic methods and instrumentation allow for identification of thousands of peptides in a single experiment. PTMScan proteomics from Cell Signaling Technology combine antibody-based enrichment with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for identification and quantitation of post-translationally modified peptides. Products and services are available for analysis of protein phosphorylation, acetylation, ubiquitination, methylation, succinylation, and caspase cleavage from cell lines, tissues, xenografts, or other biological materials (PTMScan Discovery). Services have also been designed for targeted, in-depth coverage of critical signaling pathways (PTMScan Direct). PTMScan methods have been successfully applied to biomarker discovery, drug development, and studies to gain a better understanding of cellular signaling.

Multiplexed Quantitative Ubiquitylome Analysis utilizing Ubiquitin Remnant Immunoprecipitation, nLC-MS/MS, and Isobaric Labels

Large-scale, multiplexed quantitative proteomics experiments have recently become more accessible due to advances in both mass spectrometry instrumentation and reagents. Today, by utilizing isobaric labels (e.g., tandem mass tags), >8,000 proteins can be routinely quantified across 10 experimental samples in 1.5 days of analysis. However, to date, the analysis of the cellular ubiquitylome has been limited in the number of samples that can be multiplexed in one experiment. In this webinar I will highlight sample preparation methods including immunoprecipitation with the PTMScan ubiquitin remnant motif antibody (Cell Signaling Technology) and labeling of resulting peptides with isobaric tags. I will also discuss instrument methods (e.g., synchronous precursor selection MS3, SPS-MS3) that allow for accurate analysis of cellular ubiquitylomes. I will highlight the application of this method in which we quantified the change in >15,000 sites of ubiquitylation simultaneously across 10 quantitative channels upon treatment with Bortezomib.

To learn more about this event visit: Post Translational Modification: Antibody Enrichment for Mass Spectrometry-Based Proteomics

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global Life Sciences community. Every year thousands of industry practitioners (from pharmaceutical & biotech companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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