SOURCE: Xtalks

Xtalks Webinars

October 23, 2014 07:05 ET

Probe-Based qPCR Approaches for Detection of SNPs and Validation of Molecular Diagnostics, Live Webinar Presented by Biosearch Technologies and Hosted by Xtalks

TORONTO, ON--(Marketwired - October 23, 2014) - In this 2-part webinar, qPCR experts describe their experience overcoming challenges to assay design for diverse applications. The BHQplus® probe format presents a compact oligo design offering enhanced specificity for the detection of challenging target sequences and discrimination of sequence variations. The live broadcast takes place on Thursday, October 30, 2014 at 1pm EDT.

Part 1: A Multiplex Two-Color Real-Time PCR Method for Quality-controlled Molecular Diagnostic Testing of FFPE Samples

James C. Willey, George Isaac Professor for Cancer Research, University of Toledo Health Sciences Campus, Consultant for Accugenomics, Inc.

The presentation will benefit:
Clinical molecular diagnostic labs, molecular diagnostic development labs (academic and industry)

Take away points:
Increasingly, cancers are diagnosed through FFPE treated cytologic material from fine needle aspirates. Thus, having methods with the necessary sensitivity and quality control to reliably measure FFPE cytologic samples is increasingly important.

Part 2: BHQplus® Probes and the Array Tape® Platform: A Successful Combination for Accurate and Economical SNP Genotyping

Cassie Keppel, Laboratory Operations Manager, Douglas Scientific

The presentation will benefit:
Laboratories that perform, or are interested in performing, probe-based SNP genotyping or fluorescence-based end-point PCR on any scale

Take away points:

  • The Array Tape platform is a flexible solution that works with a variety of applications and with many chemistry options, including assays, master mixes, and sample types.
  • The Array Tape platform from Douglas Scientific and SNP genotyping assays from Biosearch Technologies are a great combination, and together provide accurate, reliable results while producing significant savings on a cost per data point basis.
  • SNP genotyping with hybridization probes require two primers and two allele-specific probes, each with a different dye label. Probe hybridization is accurate and produces correct calls, but it is not 100% present or absent based on alleles.
  • Oligos and assays, including the software to generate them, differ between oligo manufacturers. They are not necessarily interchangeable, and may respond differently to PCR conditions.
  • SNP assay design is simple and convenient with Biosearch Technologies software and support.

To learn more about this event visit: Probe-based qPCR Approaches for Detection of SNPs and Validation of Molecular Diagnostics

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global Life Sciences community. Every year thousands of industry practitioners (from pharmaceutical & biotech companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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