SOURCE: Xtalks

Xtalks Webinars

June 27, 2016 08:19 ET

Upcoming Webinar on Pushing the Limits of PCR: Considerations for Assay Development in High-Throughput qPCR and Digital PCR Platforms

TORONTO, ON--(Marketwired - June 27, 2016) - On Wednesday, July 13, 2016, Xtalks will host a complimentary webinar featuring Luke Linz, Laboratory Operations Manager at LGC Douglas Scientific, followed by Jim Huggett, Principal Scientist of Nucleic Acid Research at LGC and Senior Lecturer of Analytical Microbiology at University of Surrey as the keynote speakers.

Part 1: Robust Assay Development for Multiplex qPCR and SNP Genotyping in Miniaturized Volumes on a High-Throughput Instrument.

Quantitative real-time PCR (qPCR) is one of the most valuable tools in biological research and is utilized in a wide range of applications including detection of Single Nucleotide Polymorphisms (SNPs), nucleic acid quantification, and gene expression analysis. Advancements in genomic sequencing technologies have expanded the overall amount of genetic information available, subsequently creating a need for laboratory instrumentation and reagents that enable accurate and cost-effective PCR analysis for routine genetic studies.

BHQplus® probes from LGC Biosearch Technologies are compatible with many commercially available master mixes and can be used in real-time and endpoint PCR platforms, including the Array Tape® Platform. A popular choice for PCR-based SNP analysis, BHQplus probes are industry-leading in quality, specificity, and economic value. The IntelliQube® is the latest evolution of the Array Tape technology, integrating liquid handling with qPCR analysis in miniaturized reaction volumes. The variety of fluorophores available for BHQplus probes align well with IntelliQube's five fluorescence channels, providing users the added capability of multiplexing SNP genotyping assays.

Reverse transcription-qPCR (RT-qPCR) is widely used for gene expression analysis, RNA virus detection, or RNA quantification experiments. RT-qPCR can be performed directly from RNA in a one-step process, or using a two-step process where RNA is first converted to cDNA and analyzed by PCR in a subsequent reaction. In both cases, BHQ® probes and the IntelliQube offer a powerful combination for generating RT-qPCR data enabling reproducibility, sensitivity, and flexibility in terms of fluorophore choice and multiplexing capability.

This webinar will discuss the use of BHQ and BHQplus probes in combination with the IntelliQube for gene expression analysis and SNP genotyping. Using samples from a variety of research markets, a demonstration will be done to illustrate successful SNP genotyping in a multiplexed format utilizing four fluorescent probes. The speakers will also assess the use of BHQ probes for gene expression analysis, briefly discussing experimental design considerations while highlighting multiplexing capabilities. Combined with the use of BHQ and BHQplus probes and the automated workflow of Array Tape, the IntelliQube proves to be a versatile and powerful platform for a variety of PCR applications.

Part 2: Digital PCR as a Tool to Support the Molecular Diagnosis of Infectious Diseases

Jim Huggett will briefly introduce digital PCR (dPCR) and then discuss the application of the methods to infectious disease analysis. The talk will describe sources of error and explore the methods reproducibility and will use a number of examples from Jim's group at LGC, which forms part of the UK's Designated National Measurement Institute for Bioanalytical measurement, to demonstrate the potential of this powerful technique. Technical problems will also be highlighted and the potential role for dPCR in the clinical analysis of infectious diseases such as measuring drug resistance or to support established methods like qPCR will also be discussed.

For more information or to register for this free webinar visit: Pushing the Limits of PCR: Considerations for Assay Development in High-Throughput qPCR and Digital PCR Platforms

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global Life Sciences community. Every year thousands of industry practitioners (from pharmaceutical & biotech companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

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